Check Linux release ls /etc/*release ## /etc/centos-release /etc/os-release /etc/redhat-release /etc/system-release cat /etc/redhat-release ## CentOS Linux release 7.3.1611 (Core) Install R # Install Extra Packages for Enterprise Linux repository configuration # R belongs to RHEL Extra Packages for Enterprise Linux (EPEL) # Extra Packages for Enterprise Linux (or EPEL) is a Fedora Special Interest Group that creates, maintains, and manages a high quality set of additional packages for Enterprise Linux sudo yum install -y epel-release sudo yum update -y sudo yum install -y R Install Rstudio server ## https://www.rstudio.com/products/rstudio/download-server/ wget https://download2.rstudio.org/rstudio-server-rhel-1.0.143-x86_64.rpm yum install --nogpgcheck rstudio-server-rhel-1.0.143-x86_64.rpm ## By default RStudio Server runs on port 8787 and accepts connections from all remote clients. After installation you should therefore be able to navigate a web browser to the fol...
An Introduction to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying previously unknown genes that contribute to a phenotype. At their simplest level, pooled libraries are single preparations of many different plasmids. Plasmids within a given library have the same backbone, but they express or target different genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting sequence, but there are multiple sequences targeting each gene in the overall library. Barcoding libraries contain plasmids with unique, semi-random sequences that can be used for applications like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen can help you begin to understand what genes ...
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