Error message: Traceback (most recent call last): File "read_google_sheet.py", line 20, in <module> wks = gc.open_by_key("1jUJOlICOH6VnyuALdU2VAc_Ci_ZVwdW-xc5kr_qkJHY") File "/usr/lib/python2.7/site-packages/gspread/client.py", line 113, in open_by_key raise SpreadsheetNotFound gspread.exceptions.SpreadsheetNotFound Solved : In the json file, there is a "client_email". You need to share your file with this email.
We got error message shown as below: [/home/omicsacademy/miniconda3] >>> PREFIX=/home/omicsacademy/miniconda3 Unpacking payload ... concurrent.futures.process._RemoteTraceback: ''' Traceback (most recent call last): File "concurrent/futures/process.py", line 368, in _queue_management_worker File "multiprocessing/connection.py", line 251, in recv TypeError: __init__() missing 1 required positional argument: 'msg' ''' The above exception was the direct cause of the following exception: Traceback (most recent call last): File "entry_point.py", line 69, in <module> File "concurrent/futures/process.py", line 484, in _chain_from_iterable_of_lists File "concurrent/futures/_base.py", line 611, in result_iterator File "concurrent/futures/_base.py", line 439, in result File "concurrent/futures/_base.py", line 388, in __get_result concurrent.futures.process.BrokenProc
• 启动子 : RNA 聚合酶特异性识别和结合的 DNA 序列。 • promoter 自然不属于 intron 和 Exon 的任何一个,属于 noncodingsequence 。 • noncoding RNA 是现在研究的热点之一。我们常见的 MiRNA,SiRNA ,antisense RNA tech, 这些都是属于 ncRNA 的范围。只要你在进一步问下:这些 RNA 是哪里来的?你就知道部分答案,跟那些看似跟编码蛋白没有关系的 DNA 序列有关系。这部分 DNA 有个统称就 junk DNA ,垃圾DNA或者冗余 DNA ,他们编码的 RNA 就属于 ncRNA . RNAi 就是迄今最经典的 ncRNA 功能典范。 • An exon is asequence of DNA that is expressed (transcribed) into RNA and then often, butwith many noteworthy exceptions[1] , translated into protein. Adjacent exons maybe separated by an intron , which is later removed from the RNAtranscript via the splicing mechanism. (From Wikipedia) • UTR ( UntranslatedRegions) 即非翻译区,是信使 RNA ( mRNA )分子两端的非编码片段。 • 5'-UTR 从 mRNA 起点的甲基化鸟嘌呤核苷酸帽延伸至 AUG 起始密码子, 3'-UTR 从编码区末端的终止密码子延伸至多聚 A 尾巴( Poly-A )的末端。 转录(Transcription)是遗传信息从DNA到RNA的转移。即以双链DNA中的一条链为模板,以ATP、CTP、GTP和UTP4种核苷三磷酸为原料,在RNA聚合酶催化下合成RNA的过程。 常见问题: 问: Promoter 在 DNA 序列中是算内含子还是外显子? 答:都不是。 属于 noncoding sequen
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